Can be used for lysing red blood cells and for fixing leukocytes in one step, allowing investigators to rapidly inactivate kinases, phosphatases and/or proteases, so that the in vivo phosphorylation state of the cell can be examined
Has been reported to preserve the light scattering properties of the cell
May be used on human and mouse whole blood
Whole blood lysis has been shown to be as effective as density gradient centrifugation in the preparation of PBMCs for lymphocyte subset analysis
In many cases, phospho-specific antibodies may be used simultaneously with the staining of cell surface markers (e.g. CD markers); however, not all cell surface markers, however, are compatible with the BD Phosflow Lyse/Fix Buffer
WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov
McKesson #
1076418
Manufacturer #
558049
Brand
Phosflow
Manufacturer
Becton Dickinson
Country of Origin
United States
Application
Immunology Reagent
Chemical Name
Lysis Buffer
Concentration Level
5X
Container Type
Bottle
For Use With
For use with Flow Cytometer
Form
Solution
Product Dating
McKesson Acceptable Dating: we will ship >= 90 days
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