Edvotek Western Blot Educational Kit 6 blots, 2.5 hours Time Range, Refrigerated

Features

• Proteins are separated on a polyacrylamide gel and are transferred (blotted) to a nylon membrane
• Membrane is exposed to solutions containing primary antibody, followed by a secondary antibody coupled to an enzyme
• Membrane is then soaked in a substrate solution to develop the color reaction to identify the antigen protein band
• Molecular weights of the visible bands are measured using prestained protein markers of known molecular weight
• Experiment times: Electrophoresis, one hour, blot, overnight, detection, two hours and 30 minutes
• Includes: Instructions, negative control, all samples and antibodies, various reagents and buffers, membrane and filter paper