Harris Hematoxylin is suitable for visualization of nuclei in tissue sections and cell preparations.This reagent contains alcohol and is suitable for use with all chromogens commonly used in immunohistochemical (IHC) applications. It may also be used for routine Hematoxylin and Eosin staining. Hematoxylin solutions contain hematein and a metal mordant. Harris Hematoxylin is a regressive stain which requires acid wash after the hematoxylin step. Slides are left in Hematoxylin solution for 8-10 minutes to stain the nuclei followed by destaining with 1% acid alcohol for 2-3 seconds or gentle dip. Then, keep the slides in running tap water or ammonia solution for bluing.
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