The identification of Mycobacteria with rhodamine-auramine is due to the affinity of the mycolic acid in the cell walls for the fluorchromes. These dyes will bind to the Mycobacteria, which appear bright yellow or orange against a greenish background. The potassium permanganate helps prevent non-specific fluorescence. All acid-fast organisms will be stained by rhodamine-auramine, including the sporozoan parasites. Slides stained with rhodamine-auramine may be restained with Ziehl-Neelsen or Kinyoun stain directly, as long as the oil has been removed. This provides a convenient method of confirming and differentiating morphology of positive slides with the traditional stains.
Auramine-Rhodamine Stain Kit
Kit includes one 8 oz. bottle of each of the following:
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