Hardy Diagnostics HardyDisks Nitrate are for the differentiation of anaerobes based on the ability to reduce nitrate
The HardyDisk Nitrate detects the nitrite that organisms produce by the colored end product that is formed after addition of nitrate reagents
Nitrate, when combined with sulfanilic acid and N,N-dimethyl-alpha-naphthylamine forms a red compound, p-sulfobenzene-azo-napthylamine, which is indicative of a positive reaction
If there is no color production (a negative reaction) zinc dust is added to confirm the negative reaction
HardyDisks Nitrate are impregnated with potassium nitrate and sodium molybdate
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